The 3 in 1 Renal Function Test Strip use a timed-endpoint method to measure the Uric Acid (UA),Creatinine(CR) and Urea (UR) concentrations in whole blood, serum or plasma. The system monitors the change in absorbance at 620 nm at a fixed-time interval. The change in absorbance is directly proportional to the concentration of metabolite in the specimen.
Peroxidase catalyzes the reaction of hydrogen peroxide with 4-aminoantipyrine and phenol to
CR: Creatinine in the specimen are hydrolyzed to creatine by the action of creatininase. A sequence of three coupled enzymatic steps using creatinase, sarcosine oxidase, and peroxidase causes the oxidative coupling of 4-aminoantipyrine to form a blue dye.
UR: Urea in the specimen are hydrolyzed to NH 3 and CO 2 by the action of urease,and an indicator layer containing an indicator which produces a detectable change by gaseous ammonia.
